MOLECULAR EPIDEMIOLOGICAL STUDY ON PESTE DES PETITS RUMINANTS IN EGYPT 2015
DOI:
https://doi.org/10.26873/SVR-237-2018Abstract
Peste des petits ruminants (PPR) is one of highly contagious viral diseases of small ruminants with high economic losses due to the high morbidity and mortality. In Egypt, PPR in the last 10 years re-emerged again with high mortality in sheep flocks. There is no much data regarding the circulating Peste des petits ruminants’ virus and the epidemiological distribution in small ruminants. The study was carried out on a sheep flock of 50 rams aged of 9-12 months with clinical signs suggestive to PPR infection (fever, erosions of the buccal mucosa, pneumonia, diarrhea high morbidity and mortality). Buffy coat and lymph nodes from diseased and dead animals were sent for diagnosis and molecular diagnosis was confirmed using RT-PCR with specific primers targeting three genes; nucleo-capsid (N), fusion (F), and hemagglutinin (H). Sequencing and phylogenetic analysis was carried out on the PCR products and revealed that, the circulating virus is belonged to lineage IV along with Ethiopian strain.
Key words: peste des petits ruminants; molecular; epidemiological; diagnosis; Egypt
MOLEKULARNA EPIDEMIOLOŠKA ŠTUDIJA KUGE DROBNICE V EGIPTU LETA 2015
Povzetek: Kuga drobnice (PPR; iz angl. peste des petits) je ena izmed zelo nalezljivih virusnih bolezni malih prežvekovalcev, ki zaradi visoke obolevnosti in umrljivosti povzroča visoke gospodarske izgube. V Egiptu se je v zadnjih desetih letih PPR znova pojavila z visoko smrtnostjo pri ovcah. O kugi drobnice in epidemiologiji te bolezni pri malih prežvekovalcih v literaturi ne najdemo veliko podatkov. Opisana raziskava je bila opravljena v čredi 50 ovnov, starih od 9 do 12 mesecev, s kliničnimi znaki, ki so kazali na prisotnost bolezni PPR (zvišana telesna temperatura, erozije ustne sluznice, pljučnica, driska, visoka obolevnost in smrtnost). Za natančno diagnosticiranje bolezni je bil uporabljen del krvi, pridobljen s centrifugiranjem, ki vsebuje veliko levkocitov in trombocitov (angl. Buffy coat) in bezgavke obolelih in mrtvih ovnov. Z uporabo specifičnih oligonukleotidov za določanje prisotnosti nukleotidne (N), fuzijske (F) in hemaglutininske (H). beljakovine v reakciji RT PCR smo na molekularnem nivoju potrdili prisotnost okužbe s povzročiteljem PPR. Analiza zaporedja genov in filogenetske analize so bile izvedene na produktih analize PCR, pri tem pa smo ugotovili, da virus pripada liniji IV skupaj z etiopijskim sevom.
Ključne besede: kuga drobnice; molekularna diagnostika; epidemiologija; Egipt
References
(1) Banyard AC, Parida S, Batten C, Oura C, Kwiatek O, Libeau G. Global distribution of peste des petits ruminants virus and prospects for improved diagnosis and control. J Gen Virol 2010; 91(12): 2885–7.
(2) Kumar KS, Babu A, Sundarapandian G, et al. Molecular characterisation of lineage IV peste des petits ruminants virus using multi gene sequence data. Vet Microbiol 2014; 174(1/2): 39-49.
(3) Roeder P, Obi T. Recognizing Peste des petits ruminants: a field manual. Rome : FAO, EMPRES, 1999: 28 p.
(4) EFSA Panel on Animal Health and Welfare. Scientific Opinion on peste des petits ruminants. EFSA Journal. 2015;13(1): e3985 (94 p.) 1. https://efsa.onlinelibrary.wiley.com/doi/pdf/10.2903/j.efsa.2015.3985
(5) Sharma A, Kumar A, Sharma P. Goat and sheep plague a fatal disease of small ruminants Livest Int 2007; 11(6): 2.
(6) Tariq A. An easy approach towards diagnosis of Peste des petits ruminants (PPR) through clinical examination. Res J Vet Pract 2014; 2(1S): 1–3.
(7) Kwiatek O, Ali YH, Saeed IK, et al. Asian lineage of Peste des petits ruminants virus, Africa. Emerg Infect Dis 2011; 17(7): 1223–31.
(8) Libeau G, Diallo A, Parida S. Evolutionary genetics underlying the spread of peste des petits ruminants virus. Anim Front 2013; 4(1): 14–20.
(9) Muniraju M, Mahapatra M, Ayelet G, et al. Emergence of lineage IV Peste des petits ruminants virus in Ethiopia: complete genome sequence of an Ethiopian isolate 2010. Transbound Emerg Dis. 2016; 63(4): 435–42.
(10) Jackson P, Cockcroft P. Clinical examination of farm animals. Oxford : Blackwell Publishing, 2002: 450 p.
(11) Stevenson M. An introduction to veterinary epidemiology. Palmerston North : EpiCentre, IVABS, Massey University, 2005: 90 p.
(12) Tariq A, Aqil K, Akbar Z, et al. Peste des petits ruminants (PPR) in small ruminants: a clinical, haemato-serological and pathological aspects. Int J Vet Sci 2014; 3(4 ): 206– 9.
(13) Abd El-Rahim I, Sharawi S, Barakat M, El-Nahas E. An outbreak of peste des petits ruminants in migratory flocks of sheep and goats in Egypt in 2006. Rev Sci Techn 2010; 29: 655–62.
(14) Soltan MA, Abd-Eldaim MM. Emergence of peste des petits ruminants virus lineage IV in Ismailia Province, Egypt. Infect Genet Evol 2014; 28: 44–7.
(15) Parida S, Muniraju M, Mahapatra M, Muthuchelvan D, Buczkowski H, Banyard AC. Peste des petits ruminants. Vet Microbiol 2015; 181(12): 90–106.
(16) Tibbo M, Woldemeskel M, Gopilo A. An outbreak of respiratory disease complex in sheep in Central Ethiopia. Trop Anim Health Prod 2001; 33(5): 355–65.
(17) Cebra C, Cebra M. Diseases of the hematologic, immunologic, and lymphatic systems (Multisystem diseases). In: Pugh DG, Baird AN, eds. Sheep and goat medicine. 2nd ed. St Louis : W.B. Saunders, 2012: 466–502.
(18) El Arbi AS, El Mamy AB, Salami H, et al. Peste des petits ruminants virus, Mauritania. Emerg Infect Dis 2014; 20(2): 334–6.
(19) Chowdhury EH, Bhuiyan AR, Rahman MM, Siddique MSA, Islam MR. Natural peste des petits ruminants virus infection in Black Bengal goats: virological, pathological and immunohistochemical investigation. BMC Vet Res 2014; 10: e263. https://bmcvetres.biomedcentral.com/articles/10.1186/s12917-014-0263-y
(20) Shaila MS, Shamaki D, Forsyth MA, et al. Geographic distribution and epidemiology of peste des petits ruminants viruses. Virus Res 1996; 43(2): 149–53.
(21) Kerur N, Jhala MK, Joshi CG. Genetic characterization of Indian peste des petits ruminants virus (PPRV) by sequencing and phylogenetic analysis of fusion protein and nucleoprotein gene segments. Res Vet Sci 2008; 85(1): 176–83.
(22) Couacy-Hymann E, Roger F, Hurard C, Guillou JP, Libeau G, Diallo A. Rapid and sensitive detection of peste des petits ruminants virus by a polymerase chain reaction assay. J Virol Methods 2002; 100(1/2): 17–25.
(23) Kwiatek O, Minet C, Grillet C, et al. Peste des petits ruminants (PPR) outbreak in Tajikistan. J Comp Pathol 2007; 136(2/3): 111–9.
(24) Munir M, Zohari S, Suluku R, et al. Genetic Characterization of Peste des petits ruminants virus, Sierra Leone. Emerg Infect Dis 2012; 18(1): 193–9.
