PRIMARY PULMONARY MYCOBACTERIOSIS IN A CAT: IMMUNOHISTOCHEMICAL AND HISTOPATHOLOGICAL EVALUATION
DOI:
https://doi.org/10.26873/SVR-1823-2024Keywords:
feline mycobacteriosis, immunohistochemistry, histopathology, pulmonaryAbstract
In this case report, primary pulmonary mycobacteriosis in a domestic cat was assessed using histopathological and immunohistochemical methods. The cat was admitted to a private veterinary clinic and presented with hyporexia, lethargy, and dyspnea with an abdominal component. For diagnostic purposes, thorax radiography was performed. Radiographic lung imaging showed the presence of opaque foci that were widely distributed throughout the lung. The cat’s condition gradually deteriorated, and the cat died 2 hours after being brought to the clinic. Following owner consent, gross post-mortem examination was performed immediately after death at the same clinic. White foci measuring 2–10 mm in diameter covered the surface of the lungs and were present throughout the parenchyma, mostly multifocally and occasionally coalescing. No other macroscopical pathological findings were observed in other organs or on the skin. Some of the lung tissue with macroscopic lesions underwent further histopathological and immunohistochemical examination. Multifocal to coalescing areas of caseous necrosis (without a fibrous capsule) with, karyorrhectic nuclei, and numerous macrophages were observed. Ziehl-Neelsen staining revealed many acid-fast bacteria. Immunohistochemical staining revealed positive immunostaining for Mycobacterium spp. This case study highlights the importance of considering zoonotic risks in cats diagnosed with primary pulmonary mycobacteriosis.
Primarna pljučna mikobakterioza pri mački: imunohistokemična in histopatološka ocena
Izvleček: V tem poročilu o primeru je bila ocenjena primarna pljučna mikobakterioza pri domači mački z uporabo histopatoloških in imunohistokemičnih metod. Mačka je bila sprejeta v zasebno veterinarsko kliniko zaradi simptomov hiporeksije, letargije in dispneje z abdominalno komponento. Za diagnostične namene je bila opravljena rentgenska preiskava prsnega koša, ki je pokazala prisotnost motnih žarišč, široko razporejenih po pljučih. Stanje mačke se je postopoma slabšalo, umrla je dve uri po prihodu v kliniko. Po privolitvi lastnika je bila takoj po smrti opravljena raztelesba. Bela žarišča, premera od 2 do 10 mm, so prekrivala površino pljuč. Prisotna so bila po celotnem parenhimu večinoma multifokalno in mestoma zlivajoče. V drugih organih in na koži ni bilo opaznih drugih makroskopskih patoloških sprememb. Nekatere dele pljučnega tkiva z makroskopskimi spremembami smo dodatno histopatološko in imunohistokemično pregledali. Opazili smo multifokalna do zlivajoča se območja kazeozne nekroze (brez fibrozne kapsule) s karioznimi jedri in številnimi makrofagi. Ziehl-Neelsenovo barvanje je razkrilo številne acidofilne bakterije. Imunohistokemično barvanje je pokazalo pozitivno imunobarvanje za Mycobacterium spp. To poročilo o primeru poudarja pomen upoštevanja zoonotskih tveganj pri mačkah z diagnozo primarne pljučne mikobakterioze.
Ključne besede: mikobakterioza mačk; imunohistokemija; histopatologija; pljuča
References
1. Sakamoto K. The pathology of Mycobacterium tuberculosis infection. Vet Pathol 2012; 49(3): 423–39. doi: 10.1177/0300985811429313
2. O'Reilly LM, Daborn C. The epidemiology of Mycobacterium bovis infections in animals and man: a review. Tuber Lung Dis 1995; 76(Suppl. 1): 1–46. doi: 10.1016/0962-8479(95)90591-x
3. Schrenzel M, Nicolas M, Witte C, et al. Molecular epidemiology of Mycobacterium avium subsp. avium and Mycobacterium intracellulare in captive birds. Vet Microbiol 2008; 126(1/3): 122–31. doi: 10.1016/j.vetmic.2007.06.016
4. Gunn‐Moore D, Dean R, Shaw S. Mycobacterial infections in cats and dogs. In Pract 2010; 32(9): 444–52. doi: 10.1136/inp.c5313
5. Rufenacht S, Bogli-Stuber K, Bodmer T, et al. Mycobacterium microti infection in the cat: a case report, literature review and recent clinical experience. J Feline Med Surg 2011; 13(3): 195–204. doi: 10.1016/j.jfms.2011.01.012
6. Palmer M. Mycobacterium bovis: characteristics of wildlife reservoir hosts. Transbound Emerg Dis 2013; 60(Suppl. 1): 1-13. doi: 10.1111/tbed.12115
7. Monies R, Cranwell M, Palmer N, Inwald J, Hewinson RG, Rule B. Bovine tuberculosis in domestic cats. Vet Rec 2000; 146(14): 407–8. doi: 10.1136/vr.146.14.407
8. Oztomurcuk D, Terzi O, Demirci C, Kılıçaslan Z. Investigation of granulomatous inflammations in terms of tuberculosis diagnosis: A 5-year multi-center laboratory study. Turk Thorac J 2022; 23(1): 11–6. doi: 10.5152/TurkThoracJ.2022.20314
9. Maxie G ed. Jubb, Kennedy & Palmer's Pathology of Domestic Animals Pathology of domestic animals. Vol. 1. St. Louis: Elsevier, 2016: 639–41.
10. Cardona P-J. The key role of exudative lesions and their encapsulation: lessons learned from the pathology of human pulmonary tuberculosis. Front Microbiol 2015; 6: 612. doi: 10.3389/fmicb.2015.00612
11. Jennings AR. The distribution of tuberculous lesions in the dog and cat, with reference to the pathogenesis. Vet Rec 1949; 61: 380–4.
12. Gunn-Moore DA, McFarland SE, Brewer JI, et al. Mycobacterial disease in cats in Great Britain: I. Culture results, geographical distribution and clinical presentation of 339 cases. J Feline Med Surg 2011; 13(12): 934–44. doi: 10.1016/j.jfms.2011.07.012
13. Miller M, Fales W, McCracken W, et al. Inflammatory pseudotumor in a cat with cutaneous mycobacteriosis. Vet Pathol 1999; 36(2): 161–3. doi: 10.1354/vp.36-2-161
14. O’Halloran C, Ioannidi O, Reed N, et al. Tuberculosis due to Mycobacterium bovis in pet cats associated with feeding a commercial raw food diet. J Feline Med Surg 2019; 21(8): 667–81. doi: 10.1177/1098612X19848455
15. Madarame H, Saito M, Ogihara K, et al. Mycobacterium avium subsp. hominissuis menigoencephalitis in a cat. Vet Microbiol 2017; 204: 43–5. doi: 10.1016/j.vetmic.2017.04.008
16. Stavinohova R, O’Halloran C, Newton JR, et al. Feline ocular mycobacteriosis: clinical presentation, histopathological features, and outcome. Vet Pathol 2019; 56(5): 749–60. doi: 10.1177/0300985819844819
17. Bennett AD, Lalor S, Schwarz T, Gunn-Moore DA. Radiographic findings in cats with mycobacterial infections. J Feline Med Surg 2011; 13(10): 718–24. doi: 10.1016/j.jfms.2011.06.006
18. Glaziou P, Floyd K, Raviglione M. Global burden and epidemiology of tuberculosis. Clinics in Chest Medicine 2009; 30: 621–36.
19. LoBue P, Enarson D, Thoen C. Tuberculosis in humans and animals: an overview. Int J Tuberc Lung Dis 2010; 14(9): 1075–8.
20. Shiloh MU, Champion PAD. To catch a killer. What can mycobacterial models teach us about Mycobacterium tuberculosis pathogenesis? Curr Opin Microbiol 2010; 13(1): 86–92. doi: 10.1016/j.mib.2009.11.006
21. McIntosh D. Feline leprosy: a review of forty-four cases from Western Canada. Can Vet J 1982; 23(10): 291–5.
22. Baral RM, Metcalfe SS, Krockenberger MB, et al. Disseminated Mycobacterium avium infection in young cats: overrepresentation of Abyssinian cats. J Feline Med Surg 2006; 8: 23–4. doi: 10.1016/j.jfms.2005.06.004
23. Horne KS, Kunkle GA. Clinical outcome of cutaneous rapidly growing mycobacterial infections in cats in the south-eastern United States: a review of 10 cases (1996–2006). J Feline Med Surg 2009; 11(8): 627–32. doi: 10.1016/j.jfms.2008.10.008
24. Smith N, Crawshaw T, Parry J. Mycobacterium microti: more diverse than previously thought. J Clin Microbiol 2009; 47(8): 2551-9.
25. Barry M, Taylor J, Woods JP. Disseminated Mycobacterium avium infection in a cat. Can Vet J 2002; 43(5): 369–71.
26. Pesciaroli M, Alvarez J, Boniotti M, et al. Tuberculosis in domestic animal species. Res Vet Sci 2014; 97(Suppl.): S78–85. doi: 10.1016/j.rvsc.2014.05.015
27. Luna L. Manual of histologic staining methods of the Armed Forces Institute of Pathology. 3rd ed. New York: McGraw-Hill Book Company, 1968: 32.
28. Ates MB, Ciftci MK, Oruc E, et al. Mycobacterium bovis tuberculosis in a neonatal Holstein calf. Eurasian J Vet Sci 2019; 35(3): 175–9. 10.15312/EurasianJVetSci.2019.242
29. Maxie G ed. Jubb, Kennedy & Palmer's Pathology of Domestic Animals Pathology
30. Mitchell J, Del Pozo J, Woolley C, Dheendsa R, Hope JC, Gunn-Moore DA. Histological and immunohistochemical (muco) cutaneous feline tuberculosis lesions. J Small Anim Pract 2022; 63(3): 174–87. doi: 10.1111/jsap.13386
31. Lloret A, Hartmann K, Pennisi MG, et al. Mycobacterioses in cats: ABCD guidelines on prevention and management. 10.1177/1098612X13489221
32. Ramdas KE, Lyashchenko KP, Greenwald R, Robbe-Austerman S, McManis C, Waters R . Mycobacterium bovis infection Emerg Infect Dis 2015; 21(3): 480–3. doi: 10.3201/eid2103.140715
33. O’Connor CM, Abid M, Walsh AL, et al. Cat-to-human transmission of Mycobacterium bovis, United Kingdom. eid2512.190012
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